汪志军
副研究员
DNA大分子硫修饰和蛋白组学
Email: wangzhijun@sjtu.edu.cn
Figure 1 PT dinucleotide is oxidized by H2O2 and PAA. (Xie , et al. Nucleic Acids Res. 2012)
Figure 2 Calcimycin biosynthesis gene cluster from S. chartreusis NRRL 3882. (Wu et al. Antimicrob Agents Chemother. 2011)
Figure 3 Interference by DNA in protein samples in BN-PAGE. (Liang, et al. Electrophoresis. 2009 )
发表论文:(*为通讯作者)
1. Characterization of the N-methyltransferase CalM involved in calcimycin biosynthesis by Streptomyces chartreusis NRRL 3882. Wu QL, Gou LX, Lin SJ, Liang JD, Yin J, Zhou XF, Bai LQ, An DR, Deng ZX*, Wang ZJ*. Biochimie. 2013 Apr 11.
2. Mutasynthesis of Pyrrole Spiroketal Compound by Using Calcimycin 3-Hydroxy Anthranilic Acid Biosynthetic Mutant. Gou LX, Wu QL, Lin SJ, Li XM, Liang JD, Zhou XF, An DR*, Deng ZX*, Wang ZJ*. Applied Microbiology and Biotechnology ,2013 May 12.
3. Phosphorothioate DNA as an antioxidant in bacteria. Xie XQ, Liang JD, Pu TN, Xu F, Yao F, Yang Y, Zhao YL, You DL, Zhou XF, Deng ZX*, Wang ZJ*. Nucleic Acids Research. 2012 Oct; 40(18):9115-24.
4. Characterization of the Pyrrole Polyether Antibiotic Calcimycin (A23187) Biosynthetic Gene Cluster from Streptomyces chartreusis NRRL 3882. Wu QL, Liang JD, Lin SJ, Zhou XF, Bai LQ *, Deng ZX and Wang ZJ*. Antimicrobial Agents and Chemotherapy (AAC),2011 Mar; 55(3):974-82.
5. Effective elimination of nucleic acids from bacterial protein samples for optimized blue native polyacrylamide gel electrophoresis. Liang JD, Niu QL, Xu XP, Luo YM, Zhou XF, Deng ZX*, Wang ZJ*. Electrophoresis.2009 Jul; 30(14):2454-9.
6. Localization of the ActIII actinorhodin polyketide ketoreductase to the cell wall. Xu XP#,Wang ZJ#,Fan KQ,Wang SL,Jia CJ,Han H,Ramalingam E,Yang KQ. FEMS MicrobiolLett.2008 Oct; 287(1):15-21.
7. DNA modification by sulfur: analysis of the sequence recognition specificity surrounding the modification sites. Liang JD#, Wang ZJ#, He XY, Li JL, Zhou XF,Deng ZX. Nucleic Acids Research.2007 Apr; 35(9):2944-54.
8. Sample preparation for two-dimensional blue native/SDS polyacrylamide gel electrophoresis in the identification of Streptomyces coelicolor cytoplasmic proteincomplexes. Wang ZJ, Xu XP, Fan KQ, Jia CJ, Yang KQ. J Biochem Biophys Methods.2007 Jun 10; 70(4):565-72.
9. A novel target of IscS in Escherichia coli: participating in DNA phosphorothioation. An XH, Xiong W, Yang Y, Li FH, Zhou XF, Wang ZJ, Deng ZX, Liang JD. PLoS One. 2012; 7(12):e51265.
10. Theoretical study on steric effects of DNA phosphorothioation: B-helical destabilization in Rp-phosphorothioated DNA . Zhang Y, Liang J, Lian P, Han Y, Chen Y, Bai L, Wang Z, Liang J, Deng Z, Zhao YL. J Phys Chem B.2012 Sep 6;116(35):10639-48.
11. Crystal structure of the cysteine desulfurase DndA from Streptomyces lividans which is involved in DNA phosphorothioation. Chen F, Zhang Z, Lin K, Qian T, Zhang Y, You D, He X, Wang Z, Liang J, Deng Z, Wu G. PLoS One. 2012; 7(5):e36635.
12. Structural insights into DndE from Escherichia coli B7A involved in DNA phosphorothioation modification. Hu W, Wang C, Liang J, Zhang T, Hu Z, Wang Z, Lan W, Li F, Wu H, Ding J, Wu G, Deng Z, Cao C. Cell Res.2012 Jul;22(7):1203-6.
13. Purification, crystallization and preliminary X-ray analysis of the DndE protein from Salmonella enterica serovar Cerro 87, which is involved in DNA phosphorothioation. F. Chen, K. Lin, Z. Zhang, L. Chen, X. Shi, C. Cao, Z. Wang, J. Liang, Z. Deng, G. Wu. Acta Crystallogr Sect F Struct Biol Cryst Commun.2011 Nov 1; 67(Pt 11):1440-2.
14. Oxazolomycin biosynthesis in Streptomyces albus JA3453 featuring an "acyltransferase-less" type Ipolyketide synthase that incorporates two distinct extender units. Zhao C, Coughlin JM, Ju J, Zhu D, Wendt-Pienkowski E, Zhou X, Wang Z, Shen B, Deng Z. J Biol Chem.2010 Jun 25;285(26):20097-108
15. Two pHZ1358-derivative Vectors for Efficient Gene Knockout in Streptomyces. He, Y, Wang Z, Bai L, Liang J, Zhou X, Deng Z. J. Microbiol. Biotechnol. 2010 4; 20(4): 678-682
16. DNA phosphorothioation in Streptomyces lividans: Mutational analysis of the dnd locus. Xu T, Liang J, Chen S, Wang L, He X, You D, Wang Z, Li A, Xu Z, Zhou X, Deng Z. BMC Microbiol.2009 Feb 20; 9(1):41.
17. Characterization of a hyperthermostable Fe-superoxide dismutase from hot spring. He Y, Fan K, Jia C, Wang Z, Pan W, Huang L, Yang K,Dong Z. Appl Microbiol Biotechnol. 2007 75, 367-376.
Copyright © 2016上海交通大学分子微生物学研究室
地址:上海市华山路1954号上海交通大学哲生馆(原科学馆) 电话:021-62932943