程海荣
副研究员
功能糖醇新型生物合成途径的设计与优化
Email: chrqrq@sjtu.edu.cn

 

研究方向: 

(1)功能糖醇新型生物合成途径的设计与优化。
(2)功能糖醇合成酶系的设计与优化。

(3)合成功能糖醇酵母的抗逆机制的研究。

 

   研究兴趣集中于“功能糖醇的微生物合成代谢”以及“合成功能糖醇的酵母的抗逆代谢”,主要体现在功能糖醇的生物转化与生物合成新途径的设计与优化。功能糖醇(如赤藓糖醇、木糖醇、甘露醇)在食品领域与医药合成领域具有重要的应用价值。我们通过构建新型的微生物细胞工厂来高效且经济的合成木糖醇、赤藓糖醇、甘露醇以及其它稀有糖。研究成果已经得到应用并取得良好的效果。截止到2018年9月,发表研究论文28篇,申请或获得授权专利11项;通过一项研究成果技术鉴定,9项研究成果成功技术转移并得到应用。2017年11月获得中国产学研创新成果一等奖。相关研究得到国家“863”、“973”计划以及国家自然科学基金的资助。 

 

教育经历:

2008.1-2010.3   博士后,上海交通大学
2003.09-2006.08  博士,中科院微生物研究所 

 

工作经历:

2013.01-今   上海交通大学,副研究员、博士生导师
2012.06-    上海交通大学,硕士生导师
2010.03-2012.12    上海交通大学,助理研究员
 

申请或者授权专利

1、邓子新,程海荣。从木糖母液以及生物质酸水解液提取L-阿拉伯糖的方法。专利申请号:200810040906.0。申请日:2008.7.24,公开日:2009年2月25日。
2、邓子新,程海荣。一种由卫矛醇制备D-塔格糖以及L-塔格糖的方法。授权公告号:CN101603064B。专利号:ZL200910052131.3。
3、邓子新,程海荣。一种从液体木糖醇里制备L-阿拉伯糖醇的方法。授权公告号:CN101560526B。专利号:ZL200910052132.8.
4、程海荣,邓子新。制备L-塔格糖的方法。授权公告号:101691597B。专利号:ZL200910307138.5。
5、程海荣,邓子新。基于革兰氏阳性安全微生物的透明质酸的异源合成方法。专利申请号:201010117889.3。申请日:2010年3月4日。公开日:2011年1月。
6、程海荣,吕霁烊,王犇,邓子新。从赤藓糖醇母液中提取赤藓糖醇的方法及其专用酵母菌种。申请时间:2013年5月。
7、程海荣,吕霁烊,王犇,李德春,邓子新。合成赤藓糖醇的Yarrowialipolytica酵母菌株及其用于生产赤藓糖醇的方法。申请时间:2013年5月。

8、程海荣;李莉娟;安瑾;张乐彬;王犇;李云枫。合成海藻糖的专用菌株及其用于合成海藻糖的方法,申请号:201510599925.7。

9、 程海荣;张乐彬;安瑾;李莉娟;王犇;李云枫。合成低聚果糖的专用菌株及其用于合成低聚果糖的方法,申请号201510599954.3。

10、程海荣;安瑾;张乐彬;李莉娟;王犇;李云枫。合成低聚半乳糖的专用菌株及用于合成低聚半乳糖的方法,申请号201510600511.1。

11、 程海荣;刘大文;王思绮。合成低聚异麦芽糖的解脂亚罗威酵母菌株及其合成方法,申请号201810455434.9。

 

发表论文:

1. Functional cloning and expression of an NAD-dependent D-Arabitol dehydrogenase gene from Gluconobacteroxydans in Escherichia coli. Cheng HR, Jiang N, Shen A and Feng YJ.FEMS Microbiol Lett, 2005,252 (1),35-42. (第一作者)。截止2018.9.1,被中外科学论文引用25次。

2. Extremely Rapid Extraction of DNA from Bacteria and Yeasts.Cheng HR and Jiang N. 2006. Biotechnol Lett, 2006,28(1),35-39. (第一作者) 截止2018.9.1,被中外科学论文引用304次。

3. Cloning, sequencing and characterization of the alpha-aminoadipate reductase gene (LYS2) from Saccharomycopsisfibuligera. Yan H, He P, Cheng HR, Shen A, Jiang N. Yeast, 2007, 24(3):189-199.

4. Cloning, Purification and Characterization of an NAD-Dependent D-Arabitol Dehydrogenase from Acetic Acid Bacterium, Acetobactersuboxydans. Cheng HR*, Li ZL, Jiang N, Deng ZX.Protein J, 2009,28(6):263-272.  (第一兼通讯作者)。截止2018.9.1,被中外科学论文引用17次。

5. An improved 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) reduction assay for evaluating the viability of Escherichia coli cells. Wang HW, Cheng HR, Wang FQ, Wei DZ, Wang X. J Microbiol Methods, 2010,82:330-333. 截止2018.9.1,被中外科学论文引用105次。

6. Microbiological production of L-arabitol from xylitol mother liquor. Jiang MG, Wang B, Yang L, Lin SJ, Cheng HR. J MicrobiolBiotechnol, 2011, 21(1):43-49. (通讯作者)

7. Comparison of methods for measuring viable E.coli cells during cultivation: Great differences in the early and late exponential growth phages. Wang HW, Cheng HR, Wei DZ, Wang FQ. J Microbiol Methods, 2011,84(1):140-143.

8. 用重组酶介导扩增技术快速扩增核酸。吕蓓, 程海荣, 严庆丰, 黄震巨, 沈桂芳, 张志芳, 李轶女, 邓子新,林敏, 程奇。中国科学C辑,2010,40(10):983-988。

9. Xylitol production from xylose mother liquor: a novel strategy that combines the use of recombinant Bacillus subtilis and Candida maltosa. Cheng HR, Wang B, Lv JY, Jiang MG, Lin SJ, Deng ZX. Microb Cell Fact, 2011,10:5.  (第一作者) 截止2018.9.1,被中外科学论文引用28次。

10. From shake flasks to bioreactors: survival of E. coli cells harboring pGST–hPTH through auto-induction by controlling initial content of yeast extract. Jia LH, Cheng HR, Wang HW, Luo H, Yan H. ApplMicrobiolBiotechnol, 2011,90(4):1419-1428.

11.A Novel Method to Prepare L-Arabinose from Xylose Mother Liquor by Yeast-mediated Biopurification. Cheng HR, Wang HW, Lv JY, Jiang MG, Lin SJ, Deng ZX. Microb Cell Fact, 2011,10:43. (第一作者) 被引20

12. Ammonia-containing dimethyl sulfoxide (DMSO): An improved solvent for the dissolution of formazan crystals in the MTT assay. Wang HW, Wang FQ, Tao X, Cheng HR. Analytical Biochem, 2012,421(1):324-326.(通讯作者)截止2018.9.1,被中外科学论文引用34次。

13. Characterization of streptonigrin biosynthesis reveals a cryptic carboxyl methylation and an unusual oxidative cleavage of a N-C bond. Xu F, Kong DK, He XY, Zhang Z, Han M, Xie XQ, Wang P, Cheng HR, Tao MF, Zhang L, Deng ZX, Lin SJ. J Am ChemSoc, 2013, 135(5):1739-48.

14. Functional role of β domain in the Thermoanaerobactertengcongensisglucoamylase. Li ZL, Wei PY, Cheng HR, He P, Wang     QH, Jiang N. Applied Microbiology and Biotechnology, 2013, DOI: 10.1007/s00253-013-5051-2.

15. Genetically Engineered Pichia pastoris Yeast for Conversion of Glucose to Xylitol by a Single-fermentation Process.Cheng HR, Lv JY, Wang HW, Wang B, Li ZL, Deng ZX*.Applied Microbiology and Biotechnology, 2014, 98:3539-3552. (通讯作者截止2018.9.1,被中外科学论文引用18次。

16. Improving the Expression of Recombinant Proteins in Escherichia coliBL21(DE3) Under Acetate Stress: An Alkaline pH Shift Approach. Hengwei Wang, Fengqing Wang, Wei Wang, Xueling Yao, Dongzhi Wei,Hairong Cheng, Zixin Deng. PloS ONE, 2014,9(11):e112777. (通讯作者)截止201711月,该论文受到该期刊的密切关注,被查阅12424次,被引用6次。截止201891日,被引用22次。

17. Zhang L, An J, Li L, Wang H, Liu D, Li N, Cheng HR, Deng Z. Highly Efficient Fructooligosaccharides Production by an Erythritol-Producing Yeast Yarrowialipolytica Displaying Fructosyltransferase. J Agric Food Chem. 2016,18,64(19):3828-37. doi: 10.1021/acs.jafc.6b00115.(通讯作者)。截止201891日,被引6次。

18. An J, Zhang L, Li L, Liu D, Cheng H, Wang H, Nawaz MZ, Cheng HR, Deng Z. An Alternative Approach to Synthesizing Galactooligosaccharides by Cell-Surface Display of β-Galactosidase on Yarrowialipolytica. J Agric Food Chem. 2016,18,64(19):3819-27. doi: 10.1021/acs.jafc.5b06138. (通讯作者)截止201891日,被引7次。

19. Wang H, Li L, Zhang L, An J, Cheng HR, Deng Z. Xylitol production from waste xylose mother liquor containing miscellaneous sugars and inhibitors: one-pot biotransformation by Candida tropicalis and recombinant Bacillus subtilis. Microb Cell Fact.2016,16,15(1):82. doi: 10.1186/s12934-016-0480-0. (通讯作者)截止201891日,被引7次。

20. Ning Li, Hengwei Wang, Huilin Cheng, Dawen Liu, Hairong Cheng, Zixin Deng. An Integrated Approach to Producing High-purity Trehalose from Maltose by the Yeast Yarrowialipolytica Displaying Trehalose Synthase (TreS) on the Cell Surface.J. Agric. Food Chem.2016, 64 (31), pp 6179–6187 (通讯作者)

21. Li Lijuan,Wang Hengwei,Cheng Hairong,Deng Zixin. Isomaltulose production by yeast surface display of sucrose isomerase from Pantoeadispersa on Yarrowialipolytica.Journal of Functional Foods, 2017, 32:208~217.(通讯作者)

22. Siqi Wang, Hengwei Wang, JiyangLv, Zixin Deng,  Hairong Cheng. Highly Efficient Erythritol Recovery from Waste Erythritol Mother Liquid by Yeast-mediated Biopurification. J. Agric. Food Chem.2017,65:11020-11028, Nov.doi: 10.1021/acs.jafc.7b04112.(通讯作者)截止201891日,被引2次。

23. Khan AZ, Shahid A, Cheng H, Mahboob S, Al-Ghanim KA, Bilal M, Nawaz MZ.Omics technologies for microalgae-based fuels and chemicals; challenges and opportunities. Protein Pept Lett. 2018;25(2):99-107. doi: 10.2174/0929866525666180122100722.

24. Bilal M, Nawaz MZ, Iqbal HMN, Hou J, Mahboob S, Al-Ghanim KA, Hairong C. Engineering ligninolytic consortium for bioconversion of lignocelluloses to ethanol and chemicals. Protein Pept Lett. 2018;25(2):108-119. doi: 10.2174/0929866525666180122105835.(共通讯作者)

25. Huiling Cheng, Siqi Wang, Muhammad Bilal, Xuemei Ge, Can Zhang, Patrick Fickers and Hairong Cheng. Identification, characterization of two NADPH-dependent erythrose reductases in the yeast Yarrowialipolytica and improvement of erythritol productivity using metabolic engineering.Microbial Cell Factories, 2018,17:133. (通讯作者)

26. 刘大文、程海荣*、邓子新。2018. 解脂耶氏酵母表面展示β-淀粉酶与α-葡萄糖转苷酶及一步法由淀粉合成低聚异麦芽糖。生物工程学报,2018. (通讯作者)

27、王艺颖、程海荣*。2018. 解脂耶氏酵母细胞表面展示乳糖水解酶高效水解乳糖。中国生物工程杂志,2018. (通讯作者)

28、Muhammad Bilala, Hairong Cheng*, YunjunYanb, Hafiz M.N. Iqbal*. Multi-Point Enzyme Immobilization, Surface Chemistry, and Novel Platforms: A Paradigm Shift in Biocatalyst Design. Critical Review in Biotechnology, 2018,Accepted on 6 September. 共通讯作者。

 

 

 

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