程海荣
副研究员
功能糖醇新型生物合成途径的重构与优化
Email: chrqrq@sjtu.edu.cn
Figure 1 Effects of various buffers, HCl, NaOH, and ammonia on the dissolution of formazan in DMSO. (Wang, et al. Analytical Biochemistry. 2012)
Figure 2 Possible pathways for the conversion of xylose to D-arabitol, glycerol, and ethanol by C. maltosa. (Cheng, et al. Microb Cell Fact. 2011)
Figure 3 Growth of yeast on solid medium containing different sugars. (Cheng, et al. Microb Cell Fact. 2011)
1. Functional cloning and expression of an NAD-dependent D-Arabitol dehydrogenase gene from Gluconobacter oxydans in Escherichia coli. Cheng HR, Jiang N, Shen A and Feng YJ. FEMS Microbiol Lett, 2005,252 (1),35-42.
2. Extremely rapid and efficient DNA extraction from bacteria and yeasts. Cheng HR and Jiang N. 2006. Biotechnol Lett, 2006,28(1),35-39.
3. Cloning, sequencing and characterization of the alpha-aminoadipate reductase gene (LYS2) from Saccharomycopsis fibuligera. Yan H, He P, Cheng HR, Shen A, Jiang N.Yeast, 2007, 24(3):189-199.
4. Cloning, Purification and Characterization of an NAD-Dependent D-Arabitol Dehydrogenase from Acetic Acid Bacterium, Acetobacter suboxydans. Cheng HR*, Li ZL, Jiang N, Deng ZX. Protein J, 2009,28(6):263-272.
5. An improved 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) reduction assay for evaluating the viability of Escherichia coli cells. Wang HW,Cheng HR, Wang FQ, Wei DZ, Wang X. J Microbiol Methods, 2010,82:330-333.
6. Microbiological production of L-arabitol from xylitol mother liquor. Jiang MG, Wang B, Yang L, Lin SJ, Cheng HR*. J Microbiol Biotechnol, 2011, 21(1):43-49. (通讯作者)
7. Comparison of methods for measuring viable E.coli cells during cultivation: Great differences in the early and late exponential growth phages. Wang HW, Cheng HR, Wei DZ, Wang FQ. J Microbiol Methods, 2011,84(1):140-143.
8. 用重组酶介导扩增技术快速扩增核酸。吕蓓,程海荣, 严庆丰, 黄震巨, 沈桂芳, 张志芳, 李轶女, 邓子新,林敏, 程奇。中国科学C辑,2010,40(10):983-988。
9. 木糖母液的生物净化。程海荣,邓子新。生物产业技术,2009,Z1:68-72。
10. Xylitol production from xylose mother liquor: a novel strategy that combines the use of recombinant Bacillus subtilis and Candida maltosa. Cheng HR, Wang B, Lv JY, Jiang MG, Lin SJ, Deng ZX. Microb Cell Fact, 2011,10:5.
11. From shake flasks to bioreactors: survival of E. coli cells harboring pGST–hPTH through auto-induction by controlling initial content of yeast extract. Jia LH, Cheng HR, Wang HW, Luo H, Yan H. Appl Microbiol Biotechnol, 2011,90(4):1419-1428.
12. 体外核酸快速扩增技术的发展和不断创新。吕蓓,程海荣,严庆丰,黄震巨,李轶女,罗达,沈桂芳,张志芳,邓子新,林敏,程奇。中国生物工程杂志,2011,31(3):91-96。
13. A Novel Method to Prepare L-Arabinose from Xylose Mother Liquor by Yeast-mediated Biopurification. Cheng HR, Wang HW, Lv JY, Jiang MG, Lin SJ, Deng ZX.Microb Cell Fact, 2011,10:43.
14. Ammonia-containing dimethyl sulfoxide (DMSO):An improved solvent for the dissolution of formazan crystals in the MTT assay. Wang HW, Wang FQ, Tao X,Cheng HR*. Analytical Biochem, 2012,421(1):324-326.(通讯作者)
15. Characterization of streptonigrin biosynthesis reveals a cryptic carboxyl methylation and an unusual oxidative cleavage of a N-C bond. Xu F, Kong DK, He XY, Zhang Z, Han M, Xie XQ, Wang P, Cheng HR, Tao MF, Zhang L, Deng ZX, Lin SJ. J Am Chem Soc, 2013, 135(5):1739-48.
16. Functional role of β domain in the Thermoanaerobacter tengcongensis glucoamylase. Li ZL, Wei PY, Cheng HR, He P, Wang QH, Jiang N. Applied Microbiology and Biotechnology, 2013, DOI: 10.1007/s00253-013-5051-2.
17. Genetically Engineered Pichia pastoris Yeast for Conversion of Glucose to Xylitol by a Single-fermentation Process. Cheng HR*, Lv JY, Wang HW, Wang B, Li ZL, Deng ZX*. Applied Microbiology and Biotechnology, 2013, DOI: 10.1007/s00253-013-5501-x. (*通讯作者)
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