陈实
麻省理工、哈佛大学博士后；现武汉大学教授
DNA硫修饰
Email: Shi_chen@hms.harvard

Current address:
Department of Cell Biology
Harvard Medical School
Harvard University
Boston, MA 02115
E-mail: Shi_chen@hms.harvard.edu
Tel: 001-857-998-8169

Selected Publications

1. Wang L.*, Chen S.*, Xu T., Taghizadeh K., Wishnok J., Zhou X., You D., Deng Z, Dedon P. Phosphorothioation of DNA in bacteria by dnd genes. Nature Chemical Biology, 2007, Nov; 3(11): 709-710. (*co-first author)

2. Chen S.*, Galan M. C.*, Carla Coltharp, O'Connor S. E. Redesign of a central enzyme in alkaloid biosynthesis. Chemistry & Biology, 2006 Nov; 13(11): 1137-41. (*co-first author)

3. Chen S, Huang X, Zhou X, Bai L, He J, Jeong KJ, Lee SY, Deng Z. Organizational and mutational analysis of a complete FR-008/candicidin gene cluster encoding a structurally related polyene complex. Chemistry & Biology, 2003 Nov; 10(11): 1065-76.

4. Chen S. *, Mao X.*, Shen Y., Zhou Y., Li J., Wang L., Tao X., Yang L., Wang Y., Zhou X., Deng Z., Wei D. A tailoring P450 monooxygenase gene for FR-008/candicidin biosynthesis. Applied and Environmental Microbiology, 2009, Mar; 75(6): 1778-81 (*co-first author)

5. Wang L, Chen S, Xiao X, Huang X, You D, Zhou X, Deng Z. arsRBOCT arsenic resistance system encoded by linear plasmid pHZ227 in Streptomyces sp. Strain FR-008. Applied and Environmental Microbiology, 2006 May; 72(5): 3738-3742.

6. Chen S., Zhou X.F., Jeong H.Y., Choi K.R., Lee S.Y., Deng Z. Sequence analysis of a region upstream of antibiotic FR-008 Gene cluster. Journal of Agricultural Biotechnology, 2005, Mar; 12(3): 404-405.

7. Mao X., Shen Y., Yang L., Chen S., Yang Y., Yang J., Zhu H., Deng Z., Wei D. Optimizing the medium compositions for accumulation of the novel FR-008/Candicidin derivatives CS101 by a mutant of Streptomyces sp. using statistical experimental methods. Process Biochemistry, 2007, May; 42(5): 878-883.

8. Mao X., Shen Y., Wei D., Chen S., Deng Z. Determination of Candicidin/FR-008 and Related Components in Fermentation Broth by RP-HPLC. Journal of Chinese Pharmaceutical Sciences, 2005, Feb; 14(2): 115-118.

9. Yerker N., Wu J. X., McCoy E., Galan C., Chen S., O'Connor S. E. Substrate specificity and diastereoselectivity of strictosidine glucosidase, a key enzyme in monoterpene indole alkaloid biosynthesis. Bioorganic & Medicinal Chemistry Letters, 2008, May; 18(10): 3095-3098.

10. Zhou Y., Li J., Zhu J., Chen S., Bai L., Zhou X., Wu H., Deng Z. Incomplete beta-Ketone Processing as a Mechanism for Polyene Structural Variation in the FR-008/Candicidin Complex. Chemistry & Biology, 2008, June; 15(6): 629-38.

11. Yang L., Mao X., Zhou W., Chen S., Shen Y., Deng Z., Wei D. Optimization on culture process conditions for biosynthesis of novel FR-008/candicidin derivatives by a mutant of Streptomyces sp. Chinese Journal of Antibiotics, 2008, June; 33(6): 333-337.

12. Zhou Y., Meng Q., You D., Li J., Chen S., Ding D., Zhou X., Zhou H., Bai L., Deng Z. Selective removal of the aberrant extender units by a type II thioesterase for the efficient FR-008/candicidin biosynthesis in Streptomyces sp. FR-008. Applied and Environmental Microbiology, 2008, Dec; 74(23): 7235-42.

13. Mao X., Wang F., Zhang J., Chen S., Deng Z., Shen Y., Wei D. The pH Shift and Precursor Feeding Strategy in a Low-Toxicity FR-008/Candicidin Derivative CS103 Fermentation Bioprocess by a Mutant of Streptomyces sp. FR-008. Applied Biochemistry and Biotechnology. 2009 Jan 16.

14. Xu T., Liang J., Chen S., Wang L., He X., You D., Wang Z., Li A., Xu Z., Zhou X., Deng Z. DNA phosphorothioation in Streptomyces lividans: Mutational analysis of the dnd locus. BMC Microbiology. 2009 Feb 20;9(1):41.

Honors and Awards

2008 National Natural Science Award, China
2006 Shanghai Graduate Research Excellence Award, Shanghai City government
2006 Tan Kah Kee Young Inventors' Award (Shanghai), Singapore Tan Kah Kee Foundation Copper Award
2004 First Grade Science Progress Award, Shanghai City government
2004 Shanghai government top 11 New Era Outstandings
2004 Tan Kah Kee Young Inventors' Award (Shanghai), Singapore Tan Kah Kee Foundation Silver Award
2004 Life Science Excellence Award, Shanghai, MEIJI Dairies Corp (Japan)
2004 Shanghai - Unilever Research & Development Fund scholarship