Ming Jiang
Associate Professor
Natural product biosynthesis
Email: jiangming9722@hotmail.com

 


Ming Jiang was born in Jiangsu, China in 1979. In 2001, he received his BSc from Fudan University in Chemistry. After receiving his MSc and PhD from HKUST in biochemistry and chemistry respectively, he continued his research as a postdoc in chemistry department in HKUST. In 2010, he went to United States to join Dr. Blaine Pfeifer’s group in Tufts University as a postdoc and then moved to University at Buffalo (with Dr. Pfeifer). In 2013, he came back to China and began as an Associate Professor at the School of Life Sciences & Biotechnology, Shanghai Jiao Tong University. His research interests involve the elucidation of natural product biosynthetic pathway and heterologous biosynthesis of natural product; the improvement of natural product yield and engineered biosynthesis of unnatural natural product through metabolic engineering. 


Research Interests:
1.    Natural product biosynthesis.
2.    Heterologous production of natural product.
3.    The improvement of natural product yield and engineered biosynthesis of unnatural natural products through metabolic engineering.
 

  

Figure 1. A bicarbonate cofactor modulates 1,4-dihydroxy -2-naphthoyl-coenzyme a synthase in menaquinone biosynthesis of Escherichia coli. [Jiang, et al. J. Biol. Chem., 2010] 


 

Figure 2. Catalytic mechanism of SHCHC synthase in the menaquinone biosynthesis of Escherichia coli: identification and mutational analysis of the active site residues. [Jiang, et al. Biochemistry, 2009] 


  

Figure 3. Effects of macromolecular crowding on the intrinsic catalytic efficiency and structure of enterobactin-specific isochorismate synthase. [Jiang, et al. J. Am. Chem. Soc., 2007] 
 
Selected Publications: 
1.    Chen M, Ma X, Chen X, Jiang M, Song H, Guo Z. Identification of a hotdog-fold thioesterase involved in the biosynthesis of menaquinone in Escherichia coli. J Bacteriol., 2013 Apr 5. [Epub ahead of print].
2.    Sun Y, Song H, Li J, Li Y, Jiang M, Zhou J, Guo Z.H. Structural basis of the induced-fit mechanism of 1,4-dihydroxy-2-naphthoyl coenzyme a synthase from the crotonase fold superfamily. PLoS One, 2013, 8(4):e63095. 
3.    Johnstona JM, Jiang M, Guo Z, Bakera E.N. The Structure of E. coli Native MenH and Two Active Site Mutants. Plos One, 2013 8(4):e61325.
4.    Jiang M, Zhang H, Pfeifer BA. The logic, experimental steps, and potential of heterologous natural product biosynthesis featuring the complex antibiotic erythromycin A produced through E. coli. J. Vis. Exp., 2013, (71):e4346.
5.    Jones C.H, Chen C.K, Jiang M, Fang L, Cheng C, Pfeifer B.A. Synthesis of cationic polylactides with tunable charge densities as nanocarriers for effective gene delivery. Mol. Pharm., 2013, 10(3): 1138-1145.
6.    Jiang M, Pfeifer BA. Downstream reactions and engineering in the reconstituted pathways for Taxol and other isoprenoid natural products. Appl. Microbiol. Biotechnol., 2012, 94(4): 841-849.
7.    Jiang M, Stephanopoulos G, Pfeifer BA. Toward biosynthetic design and implementation of Escherichia coli-derived paclitaxel and other heterologous polyisoprene compounds. Appl. Environ. Microb., 2012, 78(8): 2497-2504.
8.    Sun Y, Song H, Li J, Jiang M, Li Y, Zhou J, Guo Z. Active Site Binding and Catalytic Role of Bicarbonate in 1,4-Dihydroxy-2-naphthoyl Coenzyme A Synthases from Vitamin K Biosynthetic Pathways. Biochemistry, 2012, 51 (22), 4580–4589.
9.    Zhang H, Skalina K, Jiang M, Pfeifer BA. Improved E. coli erythromycin a production through the application of metabolic and bioprocess engineering. Biotechnol. Prog., 2012, 28 (1): 292-296.
10.    Chen M.J, Jiang M, Sun Y.R, Guo ZF, Guo Z. Stabilization of the second oxyanion intermediate by 1,4-dihydroxy-2-naphthoyl coenzyme A synthase of the menaquinone pathway: Spectroscopic evidence for the involvement of a conserved aspartic acid. Biochemistry, 2011, 50 (26): 5893-5904.
11.    Jiang M, Chen M, Guo ZF, Guo Z. A bicarbonate cofactor modulates 1,4-dihydroxy -2-naphthoyl-coenzyme a synthase in menaquinone biosynthesis of Escherichia coli. J. Biol. Chem., 2010, 285 (39): 30159-30169.
12.    Johnston JM, Jiang M, Guo Z, Baker EN. Structural and functional analysis of Rv0554 from Mycobacterium tuberculosis: testing a putative role in menaquinone biosynthesis. Acta Crystallogr D Biol Crystallogr., 2010, 66 (Pt 8): 909-917.
13.    Guo ZF, Jiang M, Zheng S, Guo Z. Structural change of the enterobactin synthetase in crowded solution and its relation to crowding-enhanced product specificity in nonribosomal enterobactin biosynthesis. Bioorg Med Chem Lett., 2010, 20 (13): 3855-3858.
14.    Jiang M, Chen X, Wu X.H, Chen M, Wu Y.D, Guo Z. Catalytic mechanism of SHCHC synthase in the menaquinone biosynthesis of Escherichia coli: identification and mutational analysis of the active site residues. Biochemistry, 2009, 48 (29): 6921-6931.
15.    Jiang M, Chen X, Guo ZF, Cao Y, Chen M, Guo Z. Identification and characterization of (1R,6R)-2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase in the menaquinone biosynthesis of Escherichia coli. Biochemistry, 2008, 47 (11): 3426-3434.
16.    Guo ZF, Jiang M, Zheng S, Guo Z. Suppression of linear side products by macromolecular crowding in nonribosomal enterobactin biosynthesis. Org. Lett., 2008, 10 (4): 649-652.
17.    Jiang M, Chen M, Cao Y, Yang Y, Sze K.H, Chen X, Guo Z. Determination of the stereochemistry of 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-carboxylate, a key intermediate in menaquinone biosynthesis. Org. Lett., 2007, 9 (23): 4765-4767.
18.    Jiang M, Cao Y, Guo Z.F, Chen M, Chen X, Guo Z.H. Menaquinone biosynthesis in Escherichia coli: identification of 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1- carboxylate as a novel intermediate and re-evaluation of MenD activity. Biochemistry, 2007, 46 (38): 10979-10989.
19.    Jiang M, Guo Z. Effects of macromolecular crowding on the intrinsic catalytic efficiency and structure of enterobactin-specific isochorismate synthase. J. Am. Chem. Soc., 2007, 129 (4): 730-731. 

 

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