汪志军 - 教师 - 上海交通大学分子微生物学研究室

研究人员

汪志军

副研究员
DNA大分子硫修饰和蛋白组学
Email: wangzhijun@sjtu.edu.cn

        汪志军，男，博士，副研究员，上海交通大学，生命科学技术学院，微生物代谢国家重点实验室。
        1, 呼吸为生物提供能量。而有氧代谢产生超氧化物、羟基自由基等活性氧，对生物大分子造成氧化损伤，导致衰老和疾病。生物需要清除活性氧。细菌有对DNA进行硫修饰的能力。修饰DNA可与过氧化氢发生反应。反应有两种结果：DNA断裂，或者脱硫转化成正常磷氧骨架。DNA硫修饰细菌表现出：1）对过氧化氢的抵抗能力；2）细胞内，硫化修饰的DNA直接降低了过氧化氢造成的氧化损伤程度。证明DNA的硫修饰赋予了DNA还原的化学性质，保护细菌对抗活性氧的氧化损伤。目前，正集中论证修饰DNA抗氧化的分子机理模型。
        2, 细菌可以合成各种各样的抗生素。抗生素的生物合成基因成簇排列。从合成的生物化学通路分析，相关的酶系可能以大型蛋白复合物的形式存在。钙霉素是具有结合钙离子功能的抗生素。钙霉素的生物合成基因簇中有五个I型聚酮合酶基因。分离、纯化和解析钙霉素生物合成大型蛋白复合物，可以为理解抗生素的生物合成机理提供帮助。

学习经历

2011.02-2013.02   QB3伯克利-唐氏杰出学者, 加州大学伯克利分校
2002.09-2006.12   博士, 中科院微生物所
1994.09-1997.06   硕士, 南京农业大学
1986.09-1990.06   学士, 南京农业大学

工作经历

2011.06-                上海交通大学，硕士生导师
2010.12-                上海交通大学微生物代谢国家重点实验室，副研究员
2007.01-2010.12   上海交通大学, 讲师
1997.06-2002.08    南通大学，讲师

研究方向：

一、DNA硫化修饰研究（DNA硫化修饰的抗氧化功能及其应用研究）

二、CMG复合物晶体结构的解析和复制机制的研究（抗生素生物合成蛋白复合物的结构及其功能研究）

Figure 1 PT dinucleotide is oxidized by H2O2 and PAA. (Xie , et al. Nucleic Acids Res. 2012)

Figure 2 Calcimycin biosynthesis gene cluster from S. chartreusis NRRL 3882. (Wu et al. Antimicrob Agents Chemother. 2011)

Figure 3 Interference by DNA in protein samples in BN-PAGE. (Liang, et al. Electrophoresis. 2009 )

发表论文：（*为通讯作者）

1. Characterization of the N-methyltransferase CalM involved in calcimycin biosynthesis by Streptomyces chartreusis NRRL 3882. Wu QL, Gou LX, Lin SJ, Liang JD, Yin J, Zhou XF, Bai LQ, An DR, Deng ZX*, Wang ZJ*. Biochimie. 2013 Apr 11.
2. Mutasynthesis of Pyrrole Spiroketal Compound by Using Calcimycin 3-Hydroxy Anthranilic Acid Biosynthetic Mutant. Gou LX, Wu QL, Lin SJ, Li XM, Liang JD, Zhou XF, An DR*, Deng ZX*, Wang ZJ*. Applied Microbiology and Biotechnology ，2013 May 12.
3. Phosphorothioate DNA as an antioxidant in bacteria. Xie XQ, Liang JD, Pu TN, Xu F, Yao F, Yang Y, Zhao YL, You DL, Zhou XF, Deng ZX*, Wang ZJ*. Nucleic Acids Research. 2012 Oct; 40(18):9115-24.
4. Characterization of the Pyrrole Polyether Antibiotic Calcimycin (A23187) Biosynthetic Gene Cluster from Streptomyces chartreusis NRRL 3882. Wu QL, Liang JD, Lin SJ, Zhou XF, Bai LQ *, Deng ZX and Wang ZJ*. Antimicrobial Agents and Chemotherapy (AAC)，2011 Mar; 55(3):974-82.
5. Effective elimination of nucleic acids from bacterial protein samples for optimized blue native polyacrylamide gel electrophoresis. Liang JD, Niu QL, Xu XP, Luo YM, Zhou XF, Deng ZX*, Wang ZJ*. Electrophoresis.2009 Jul; 30(14):2454-9.
6. Localization of the ActIII actinorhodin polyketide ketoreductase to the cell wall. Xu XP#，Wang ZJ#，Fan KQ，Wang SL，Jia CJ，Han H，Ramalingam E，Yang KQ. FEMS MicrobiolLett.2008 Oct; 287(1):15-21.
7. DNA modification by sulfur: analysis of the sequence recognition specificity surrounding the modification sites. Liang JD#, Wang ZJ#, He XY, Li JL, Zhou XF，Deng ZX. Nucleic Acids Research.2007 Apr; 35(9):2944-54.
8. Sample preparation for two-dimensional blue native/SDS polyacrylamide gel electrophoresis in the identification of Streptomyces coelicolor cytoplasmic proteincomplexes. Wang ZJ, Xu XP, Fan KQ, Jia CJ, Yang KQ. J Biochem Biophys Methods.2007 Jun 10; 70(4):565-72.
9. A novel target of IscS in Escherichia coli: participating in DNA phosphorothioation. An XH, Xiong W, Yang Y, Li FH, Zhou XF, Wang ZJ, Deng ZX, Liang JD. PLoS One. 2012; 7(12):e51265.
10. Theoretical study on steric effects of DNA phosphorothioation: B-helical destabilization in Rp-phosphorothioated DNA . Zhang Y, Liang J, Lian P, Han Y, Chen Y, Bai L, Wang Z, Liang J, Deng Z, Zhao YL. J Phys Chem B.2012 Sep 6;116(35):10639-48.
11. Crystal structure of the cysteine desulfurase DndA from Streptomyces lividans which is involved in DNA phosphorothioation. Chen F, Zhang Z, Lin K, Qian T, Zhang Y, You D, He X, Wang Z, Liang J, Deng Z, Wu G. PLoS One. 2012; 7(5):e36635.
12. Structural insights into DndE from Escherichia coli B7A involved in DNA phosphorothioation modification. Hu W, Wang C, Liang J, Zhang T, Hu Z, Wang Z, Lan W, Li F, Wu H, Ding J, Wu G, Deng Z, Cao C. Cell Res.2012 Jul;22(7):1203-6.
13. Purification, crystallization and preliminary X-ray analysis of the DndE protein from Salmonella enterica serovar Cerro 87, which is involved in DNA phosphorothioation. F. Chen, K. Lin, Z. Zhang, L. Chen, X. Shi, C. Cao, Z. Wang, J. Liang, Z. Deng, G. Wu. Acta Crystallogr Sect F Struct Biol Cryst Commun.2011 Nov 1; 67(Pt 11):1440-2.
14. Oxazolomycin biosynthesis in Streptomyces albus JA3453 featuring an "acyltransferase-less" type Ipolyketide synthase that incorporates two distinct extender units. Zhao C, Coughlin JM, Ju J, Zhu D, Wendt-Pienkowski E, Zhou X, Wang Z, Shen B, Deng Z. J Biol Chem.2010 Jun 25;285(26):20097-108
15. Two pHZ1358-derivative Vectors for Efficient Gene Knockout in Streptomyces. He, Y, Wang Z, Bai L, Liang J, Zhou X, Deng Z. J. Microbiol. Biotechnol. 2010 4; 20(4): 678-682
16. DNA phosphorothioation in Streptomyces lividans: Mutational analysis of the dnd locus. Xu T, Liang J, Chen S, Wang L, He X, You D, Wang Z, Li A, Xu Z, Zhou X, Deng Z. BMC Microbiol.2009 Feb 20; 9(1):41.
17. Characterization of a hyperthermostable Fe-superoxide dismutase from hot spring. He Y, Fan K, Jia C, Wang Z, Pan W, Huang L, Yang K，Dong Z. Appl Microbiol Biotechnol. 2007 75, 367-376.