蒋明 - 教师 - 上海交通大学分子微生物学研究室

研究人员

蒋明

副研究员
天然产物的合成生物学研究
Email: jiangming9722@hotmail.com

2001年获得复旦大学化学系学士学位，2004年和2008年分别获得香港科技大学生物化学系硕士学位和化学系博士学位。2008年至2013年分别在香港科技大学化学系，美国塔夫茨大学化学与生物工程系以及纽约州立大学水牛城分校化学与生物工程系从事博士后研究。2013年回国任上海交通大学生命科学技术学院副研究员。主要从事复杂天然产物的合成生物学以及异源生物合成研究；利用代谢工程的手段提高天然产物的产量，改变天然产物的结构。在JACS、JBC、Org. Lett.、AEM等杂志发表二十多篇研究论文。

教育经历:
    2005. 01 ~ 2008. 08：香港科技大学，化学专业，获博士学位
    2001. 09 ~ 2004. 08：香港科技大学，生物化学专业，获硕士学位
    1997. 09 ~ 2001. 06：复旦大学, 化学专业，获学士学位

工作经历:
    2013.05~至今：上海交通大学，生命科学技术学院，副研究员
    2011.09 ~ 2013.05: 纽约州立大学水牛城分校，化学与生物工程专业，博士后
    2010.09 ~ 2011.08: 塔夫茨大学，化学与生物工程专业，博士后
    2008.09 ~ 2010.08: 香港科技大学，化学专业，博士后

研究方向:
1.   天然产物的合成生物学研究
2.   天然产物的异源生物合成
3.   通过代谢工程手段达到天然产物产量的提高和结构的改变。

Figure 1. A bicarbonate cofactor modulates 1,4-dihydroxy -2-naphthoyl-coenzyme a synthase in menaquinone biosynthesis of Escherichia coli. [Jiang, et al. J. Biol. Chem., 2010]

Figure 2. Catalytic mechanism of SHCHC synthase in the menaquinone biosynthesis of Escherichia coli: identification and mutational analysis of the active site residues. [Jiang, et al. Biochemistry, 2009]

Figure 3. Effects of macromolecular crowding on the intrinsic catalytic efficiency and structure of enterobactin-specific isochorismate synthase. [Jiang, et al. J. Am. Chem. Soc., 2007]

代表性论文:

1.    Chen M, Ma X, Chen X, Jiang M, Song H, Guo Z. Identification of a hotdog-fold thioesterase involved in the biosynthesis of menaquinone in Escherichia coli. J Bacteriol., 2013 Apr 5. [Epub ahead of print].
2.    Sun Y, Song H, Li J, Li Y, Jiang M, Zhou J, Guo Z.H. Structural basis of the induced-fit mechanism of 1,4-dihydroxy-2-naphthoyl coenzyme a synthase from the crotonase fold superfamily. PLoS One, 2013, 8(4):e63095.
3.    Johnstona JM, Jiang M, Guo Z, Bakera E.N. The Structure of E. coli Native MenH and Two Active Site Mutants. Plos One, 2013 8(4):e61325.
4.    Jiang M, Zhang H, Pfeifer BA. The logic, experimental steps, and potential of heterologous natural product biosynthesis featuring the complex antibiotic erythromycin A produced through E. coli. J. Vis. Exp., 2013, (71):e4346.
5.    Jones C.H, Chen C.K, Jiang M, Fang L, Cheng C, Pfeifer B.A. Synthesis of cationic polylactides with tunable charge densities as nanocarriers for effective gene delivery. Mol. Pharm., 2013, 10(3): 1138-1145.
6.    Jiang M, Pfeifer BA. Downstream reactions and engineering in the reconstituted pathways for Taxol and other isoprenoid natural products. Appl. Microbiol. Biotechnol., 2012, 94(4): 841-849.
7.    Jiang M, Stephanopoulos G, Pfeifer BA. Toward biosynthetic design and implementation of Escherichia coli-derived paclitaxel and other heterologous polyisoprene compounds. Appl. Environ. Microb., 2012, 78(8): 2497-2504.
8.    Sun Y, Song H, Li J, Jiang M, Li Y, Zhou J, Guo Z. Active Site Binding and Catalytic Role of Bicarbonate in 1,4-Dihydroxy-2-naphthoyl Coenzyme A Synthases from Vitamin K Biosynthetic Pathways. Biochemistry, 2012, 51 (22), 4580–4589.
9.    Zhang H, Skalina K, Jiang M, Pfeifer BA. Improved E. coli erythromycin a production through the application of metabolic and bioprocess engineering. Biotechnol. Prog., 2012, 28 (1): 292-296.
10.    Chen M.J, Jiang M, Sun Y.R, Guo ZF, Guo Z. Stabilization of the second oxyanion intermediate by 1,4-dihydroxy-2-naphthoyl coenzyme A synthase of the menaquinone pathway: Spectroscopic evidence for the involvement of a conserved aspartic acid. Biochemistry, 2011, 50 (26): 5893-5904.
11.    Jiang M, Chen M, Guo ZF, Guo Z. A bicarbonate cofactor modulates 1,4-dihydroxy -2-naphthoyl-coenzyme a synthase in menaquinone biosynthesis of Escherichia coli. J. Biol. Chem., 2010, 285 (39): 30159-30169.
12.    Johnston JM, Jiang M, Guo Z, Baker EN. Structural and functional analysis of Rv0554 from Mycobacterium tuberculosis: testing a putative role in menaquinone biosynthesis. Acta Crystallogr D Biol Crystallogr., 2010, 66 (Pt 8): 909-917.
13.    Guo ZF, Jiang M, Zheng S, Guo Z. Structural change of the enterobactin synthetase in crowded solution and its relation to crowding-enhanced product specificity in nonribosomal enterobactin biosynthesis. Bioorg Med Chem Lett., 2010, 20 (13): 3855-3858.
14.    Jiang M, Chen X, Wu X.H, Chen M, Wu Y.D, Guo Z. Catalytic mechanism of SHCHC synthase in the menaquinone biosynthesis of Escherichia coli: identification and mutational analysis of the active site residues. Biochemistry, 2009, 48 (29): 6921-6931.
15.    Jiang M, Chen X, Guo ZF, Cao Y, Chen M, Guo Z. Identification and characterization of (1R,6R)-2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase in the menaquinone biosynthesis of Escherichia coli. Biochemistry, 2008, 47 (11): 3426-3434.
16.    Guo ZF, Jiang M, Zheng S, Guo Z. Suppression of linear side products by macromolecular crowding in nonribosomal enterobactin biosynthesis. Org. Lett., 2008, 10 (4): 649-652.
17.    Jiang M, Chen M, Cao Y, Yang Y, Sze K.H, Chen X, Guo Z. Determination of the stereochemistry of 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-carboxylate, a key intermediate in menaquinone biosynthesis. Org. Lett., 2007, 9 (23): 4765-4767.
18.    Jiang M, Cao Y, Guo Z.F, Chen M, Chen X, Guo Z.H. Menaquinone biosynthesis in Escherichia coli: identification of 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1- carboxylate as a novel intermediate and re-evaluation of MenD activity. Biochemistry, 2007, 46 (38): 10979-10989.
19.    Jiang M, Guo Z. Effects of macromolecular crowding on the intrinsic catalytic efficiency and structure of enterobactin-specific isochorismate synthase. J. Am. Chem. Soc., 2007, 129 (4): 730-731.