程海荣
研究员
功能糖醇新型生物合成途径的设计与优化
Email: chrqrq@sjtu.edu.cn

 

 

 

  • 教育与科研经历

2021.1-今        上海交通大学生命科学技术学院,研究员,博士生导师

2013.1-2020.12   上海交通大学生命科学技术学院,副研究员,2017年任博士生导师

2010.3-2012.12   上海交通大学生命科学技术学院,助理研究员

2008.1-2010.3  上海交通大学生命科学技术学院,博士后    

2003.9-2006.8  中科院微生物研究所,博士生    

2000.9-2003.8  广西大学生命科学技术学院,硕士生

1994.9-1998.7  华中农业大学园林学院(园艺系),本科生     

2016/2017/2018/2019年多次去波士顿、圣迭戈等地参加国际学术会议并做大会报告,介绍在功能糖醇领域的年度研究进展。

 

  • 研究领域:

       一直聚焦于解脂耶氏酵母的功能糖(醇)合成代谢的研究,开发高效的解脂耶氏酵母合成体系(单一途径与多酶途径合成体系)。主要通过构建新型的微生物细胞工厂,以代谢工程手段来改良解脂耶氏酵母来高效且经济合成多种功能性糖醇或者低聚糖。以绿色多酶或全细胞催化替代化学全合成。先后研究开发了赤藓糖醇的绿色高效合成菌株、功能糖醇与功能性低聚糖的级联合成技术体系、功能糖醇母液的生物净化研究、一步发酵合成法合成甘露醇与木糖醇。研究成果已经得到应用并取得良好的应用效果。申请或获得授权专利13项。同时在国内外学术期刊发表科学论文30余篇。作为项目主持人,先后承担国家“863”“973”以及国家自然科学基金项目。作为第一完成人,“功能糖醇类产品高效生物催化和减排关键技术研发及产业化”项目荣获中国产学研促进会2017年度创新成果一等奖。

 

  • 发表的论文(以下引用截止到2020.10.5,检索国内外被引

1. Functional cloning and expression of an NAD-dependent D-Arabitol dehydrogenase gene from Gluconobacter oxydans in Escherichia coli. Cheng HR, Jiang N, Shen A and Feng YJ. FEMS Microbiol Lett, 2005,252 (1),35-42.   (第一作者)。(被引27次)

2. Extremely Rapid Extraction of DNA from Bacteria and Yeasts. Cheng HR and Jiang N. 2006. Biotechnol Lett, 2006,28(1),35-39. (第一作者) 被中外科学论文引用371次。

3. Cloning, sequencing and characterization of the alpha-aminoadipate reductase gene (LYS2) from Saccharomycopsis fibuligera. Yan H, He P, Cheng HR, Shen A, Jiang N. Yeast, 2007, 24(3):189-199.

4. Cloning, Purification and Characterization of an NAD-Dependent D-Arabitol Dehydrogenase from Acetic Acid Bacterium, Acetobacter suboxydans. Cheng HR*, Li ZL, Jiang N, Deng ZX. Protein J, 2009,28(6):263-272.  (通讯作者)。被中外科学论文引用19次。

5. An improved 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) reduction assay for evaluating the viability of Escherichia coli cells. Wang HW, Cheng HR, Wang FQ, Wei DZ, Wang X. J Microbiol Methods, 2010,82:330-333. 被中外科学论文引用163次。

6. Microbiological production of L-arabitol from xylitol mother liquor. Jiang MG, Wang B, Yang L, Lin SJ, Cheng HR. J Microbiol Biotechnol, 2011, 21(1):43-49. (通讯作者)

7. Comparison of methods for measuring viable E.coli cells during cultivation: Great differences in the early and late exponential growth phages. Wang HW, Cheng HR, Wei DZ, Wang FQ. J Microbiol Methods, 2011,84(1):140-143.

8. 用重组酶介导扩增技术快速扩增核酸。吕蓓, 程海荣, 严庆丰, 黄震巨, 沈桂芳, 张志芳, 李轶女, 邓子新, 林敏, 程奇。中国科学C辑,2010,40(10):983-988。

9. Xylitol production from xylose mother liquor: a novel strategy that combines the use of recombinant Bacillus subtilis and Candida maltosa. Cheng HR, Wang B, Lv JY, Jiang MG, Lin SJ, Deng ZX. Microb Cell Fact, 2011,10:5.  (第一作者) 被中外科学论文引用41次。

10. From shake flasks to bioreactors: survival of E. coli cells harboring pGST–hPTH through auto-induction by controlling initial content of yeast extract. Jia LH, Cheng HR, Wang HW, Luo H, Yan H. Appl Microbiol Biotechnol, 2011,90(4):1419-1428.

11.A Novel Method to Prepare L-Arabinose from Xylose Mother Liquor by Yeast-mediated Biopurification. Cheng HR, Wang HW, Lv JY, Jiang MG, Lin SJ, Deng ZX. Microb Cell Fact, 2011,10:43. (第一作者) 被引35次

12. Ammonia-containing dimethyl sulfoxide (DMSO): An improved solvent for the dissolution of formazan crystals in the MTT assay. Wang HW, Wang FQ, Tao X, Cheng HR. Analytical Biochem, 2012,421(1):324-326.(通讯作者)被中外科学论文引用47次。

13. Characterization of streptonigrin biosynthesis reveals a cryptic carboxyl methylation and an unusual oxidative cleavage of a N-C bond. Xu F, Kong DK, He XY, Zhang Z, Han M, Xie XQ, Wang P, Cheng HR, Tao MF, Zhang L, Deng ZX, Lin SJ. J Am Chem Soc, 2013, 135(5):1739-48.

14. Functional role of β domain in the Thermoanaerobacter tengcongensis glucoamylase. Li ZL, Wei PY, Cheng HR, He P, Wang QH, Jiang N. Applied Microbiology and Biotechnology, 2013, DOI: 10.1007/s00253-013-5051-2.

15. Genetically Engineered Pichia pastoris Yeast for Conversion of Glucose to Xylitol by a Single-fermentation Process. Cheng HR, Lv JY, Wang HW, Wang B, Li ZL, Deng ZX*. Applied Microbiology and Biotechnology, 2014, 98:3539-3552. (通讯作者)被中外科学论文引用30次。

16. Improving the Expression of Recombinant Proteins in Escherichia coli BL21(DE3) Under Acetate Stress: An Alkaline pH Shift Approach. Hengwei Wang, Fengqing Wang, Wei Wang, Xueling Yao, Dongzhi Wei, Hairong Cheng, Zixin Deng. PloS ONE, 2014,9(11):e112777. (通讯作者)截止2020年10月5日,该论文受到该期刊的密切关注,被查阅28,998次,被引用32次。

17. Zhang L, An J, Li L, Wang H, Liu D, Li N, Cheng HR, Deng Z. Highly efficient fructooligosaccharides production by an erythritol-producing yeast Yarrowia lipolytica displaying fructosyltransferase. J Agric Food Chem. 2016,18,64(19):3828-37. doi: 10.1021/acs.jafc.6b00115. (通讯作者)。被引15次。Journal of Agricultural and Food Chemistry

18. An J, Zhang L, Li L, Liu D, Cheng H, Wang H, Nawaz MZ, Cheng HR, Deng Z. An Alternative Approach to Synthesizing Galactooligosaccharides by Cell-Surface Display of β-Galactosidase on Yarrowia lipolytica. Journal of Agricultural and Food Chemistry. 2016,18,64(19):3819-27. doi: 10.1021/acs.jafc.5b06138. (通讯作者)被引18次。

19. Wang H, Li L, Zhang L, An J, Cheng HR, Deng Z. Xylitol production from waste xylose mother liquor containing miscellaneous sugars and inhibitors: one-pot biotransformation by Candida tropicalis and recombinant Bacillus subtilis. Microbial Cell Factories 2016,16,15(1):82. doi: 10.1186/s12934-016-0480-0. (通讯作者)被引20次。

20. Ning Li, Hengwei Wang, Huilin Cheng, Dawen Liu, Hairong Cheng, Zixin Deng. An integrated approach to producing high-purity trehalose from maltose by the yeast Yarrowia lipolytica displaying trehalose synthase (TreS) on the cell surface. J. Agric. Food Chem., 2016, 64 (31):6179–6187 (通讯作者)被引17次

21. Li Lijuan,Wang Hengwei,Cheng Hairong,Deng Zixin. Isomaltulose production by yeast surface display of sucrose isomerase from Pantoea dispersa on Yarrowia lipolytica. Journal of Functional Foods, 2017, 32:208~217.(通讯作者)被引14

22. Siqi Wang, Hengwei Wang, Jiyang Lv, Zixin Deng,  Hairong Cheng. Highly Efficient Erythritol Recovery from Waste Erythritol Mother Liquor by a Yeast-Mediated Biorefinery Process. J. Agric. Food Chem., 2017, 65:11020-11028, Nov. doi: 10.1021/acs.jafc.7b04112.(通讯作者)被引8次。

23. Khan AZ, Shahid A, Cheng H, Mahboob S, Al-Ghanim KA, Bilal M, Nawaz MZ.Omics technologies for microalgae-based fuels and chemicals; challenges and opportunities. Protein Pept Lett. 2018;25(2):99-107. doi: 10.2174/0929866525666180122100722. 被引5次

24. Bilal M, Nawaz MZ, Iqbal HMN, Hou J, Mahboob S, Al-Ghanim KA, Cheng H. Engineering ligninolytic consortium for bioconversion of lignocelluloses to ethanol and chemicals. Protein Pept Lett. 2018;25(2):108-119. doi: 10.2174/0929866525666180122105835.(共通讯作者)被引15次

25. Huiling Cheng, Siqi Wang, Muhammad Bilal, Xuemei Ge, Can Zhang, Patrick Fickers and Hairong Cheng. Identification, characterization of two NADPH-dependent erythrose reductases in the yeast Yarrowia lipolytica and improvement of erythritol productivity using metabolic engineering. Microbial Cell Factories, 2018,17:133. (通讯作者) 被引8次

26. 刘大文、程海荣*、邓子新。 解脂耶氏酵母表面展示β-淀粉酶与α-葡萄糖转苷酶及一步法由淀粉合成低聚异麦芽糖。生物工程学报, 2019年01期 第121-132页。(通讯作者)

27、王艺颖、程海荣*。2018. 解脂耶氏酵母细胞表面展示乳糖水解酶高效水解乳糖。中国生物工程杂志,2018,38(8):41-49.(通讯作者)

28、Bilal M, Asgher M, Cheng H*, Yan Y, Iqbal H.M.N.*. Multi-Point Enzyme Immobilization, Surface Chemistry, and Novel Platforms: A Paradigm Shift in Biocatalyst Design. Critical Reviews in Biotechnology, 2019, 39(2), 202–219. 共通讯作者。高被引论文,发表一年半以来被引用70次,IF8.054.

29、Ping Chi, Siqi Wang, Xuemei Ge, Muhammad Bilal, Patrick Fickers, Hairong Cheng*. Efficient D-threitol production by an engineered strain of Yarrowia lipolytica overexpressing xylitol dehydrogenase gene from Scheffersomyces stipitis. Biochemical Engineering Journal.149 (2019) 107259. https://doi.org/10.1016/j.bej.2019.107259。通讯作者。被引用4次.

30、Yirong Xu,Ping Chi,Muhammad Bilal,Hairong Cheng*. Biosynthetic strategies to produce xylitol: an economical venture. Applied Microbiology and Biotechnology, 2019, 103:5143-5160. https://doi.org/10.1007/s00253-019-09881-1。通讯作者。被引用4次.

31. Muhammad Bilal*, Shuo Xu, Hafiz M.N. Iqbal, Hairong Cheng*. Yarrowia lipolytica as an emerging biotechnological chassis for functional sugars biosynthesis. Critical Reviews in Food Science and Nutrition, DOI: 10.1080/10408398.2020.1739000. Published online: 17 Mar 2020,共通讯作者。本文围绕我课题组近五年来在解脂耶氏酵母功能糖(醇)领域的应用研究,并结合国内外其他学者的研究,进行了全方位的论述与展望。

32. Patrick Fickers*, Hairong Cheng, Carol Sze Ki Lin. Sugar alcohols and organic acids synthesis in Yarrowia lipolytica: where are we? Microorganisms, invited review by MDPI, 2020, 8:574.

33、Yirong Xu, Ping Chi, Jiyang Lv, Muhammad Bilal, Hairong Cheng*. L-Xylo-3-hexulose, a new rare sugar produced by the action of acetic acid bacteria on D-galactitol, an exception to Bertrand Hudson's rule. BBA-General Subjects,  Published online: Sep26, 2020. Volume 1865, Issue 1, January 2021, 129740,通讯作者。在研究过程中,我们发现了氧化醋酸杆菌细胞膜结合的PQQ依赖的脱氢酶氧化多元醇半乳糖醇的独特的机制,并证实与现有的膜结合的PQQ依赖的脱氢酶氧化多元醇的Bertrand Hudson法则相悖。

34. Muhammad Zohaib Nawaz, Muhammad Bilal, Arslan Tariq, Hafiz M. N.Iqbal, Huda Ahmed Alghamdi & Hairong Cheng*. Bio-purification of sugar industry wastewater and production of high-value industrial products with a zero-waste concept. Critical Reviews in Food Science and Nutrition, DOI: 10.1080/10408398.2020.1802696, Published online: 21 Aug 2020. 通讯作者。

35. Wang Nan, Ping Chi, Yawen Zou, Yirong Xu, Shuo Xu, M. Bilal, Patrick Fickers, Hairong Cheng*. Metabolic engineering of Yarrowia lipolytica for thermoresistance and enhanced erythritol productivity.  Biotechnology for Biofuel, 2020, 13:176. 通讯作者。

36. 王楠,邹亚文,程海荣*。通过改组解脂耶氏酵母基因组获得无泡沫发酵合成赤藓糖醇的研究。基因组学与应用生物学,已接收(2020年6月24日)。通讯。本研究通过基因组改组,获得一株产泡性能显著降低的解脂耶氏酵母,在合成功能糖醇的过程中无需添加消泡剂或显著减少消泡剂使用量,取得良好的效果。

      A:不产泡的工程菌分别在2升摇瓶,50升与30立方发酵罐中试验,无泡。

      B:对比产泡菌分别在2升摇瓶,50升与30立方发酵罐中试验,泡沫很多。

      工程菌株自我消泡效果非常明显。

37. Hira Munir, Asima Mumtaz, Robina Rashid, Jawayria Najeeb,Muhammad Talha Zubair, Sahar Munir, Muhammad Bilal, Hairong Cheng*.Eucalyptus camaldulensis gum as a green matrix to fabrication of zinc and silver nanoparticles:Characterization and novel prospects as antimicrobial and dye-degrading agents. Journal of Materials Research and Technology, 2020,9(6):15513-15524. (共通讯作者)

 

  • 专利

1、程海荣,吕霁烊,王犇,邓子新。从赤藓糖醇母液中提取赤藓糖醇的方法及其专用酵母菌种。专利号:ZL201310283189。授权

2、程海荣,吕霁烊,王犇,李德春,邓子新。合成赤藓糖醇的Yarrowia lipolytica酵母菌株及其用于生产赤藓糖醇的方法。ZL201310382059. 授权

4. 程海荣;张乐彬;安瑾;李莉娟;王犇;李云枫。合成低聚果糖的专用菌株及其用于合成低聚果糖的方法。申请号201510599954.3。 实审,申请公布日:2016.1.6

5. 程海荣;安瑾;张乐彬;李莉娟;王犇;李云枫。合成低聚半乳糖的专用菌株及用于合成低聚半乳糖的方法。ZL201510600511.1. 授权公告日:2019.1.11

6.程海荣;刘大文;王思绮。合成低聚异麦芽糖的解脂亚罗威酵母菌株及其合成方法。申请号201810455434.9。实审,申请公布日:2018.12.28

7、程海荣。合成木糖醇的重组解脂耶氏酵母的构建方法及其菌株。申请号:2019111111632.实审,申请公布日:2020.3.13。申请公布号:CN110878261A,申请日:2019.11.14

8、程海荣,王楠,池萍。合成赤藓糖醇的重组解脂耶氏酵母菌的构建方法及其菌株。申请号:202010069250.实审,申请公布日:2020.7.3。申请日:2020.01.21

9. 程海荣,李莉娟,安瑾,张乐彬,王犇,李云枫。合成海藻糖的专用菌株及其用于合成海藻糖的方法。专利号:ZL2015105999257。授权公告日:2018.10.23,授权公告号:CN 105219663 B

10.程海荣,邹亚文。合成海藻糖的重组解脂耶氏酵母菌的构建方法。申请号:202010252783.8,申请日2020.4.1,申请公布日:2020.8.7。申请公布号:CN111500617A

 

 

 

 

 

 

 

 

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