刘天罡
武汉大学教授
生物柴油和酯杉醇
Email: liutg@whu.edu.cn

Education:
2002.6- now Prof. Deng's lab, School of Life Science & Biotechnology, Shanghai Jiaotong UniversityA. As a PHD student.B. Lead of the graduate class
2002.6-1998.9 Department of Biotechnology, Shandong University

A. the golden prize winner of the "challenge cup" among all the college students throughout China, which is the biggest honor for the college students nationwide.
B. Seven Scholarships: first grade scholarship once, second grade scholarship twice, third grade scholarship three times, GAOTONGYI scholarship once
C. the first grade winner of the SHANDA cup of the sixth cultural festival
D. bronze medal winner of the decathlon in the sports meet of Shandong University
E. bronze medal winner of the basketball game of Shandong University as the coach and Shooting Guard

Research experiences:
2002.3-now Prof. Deng Zixin's labThe mechanism of biosynthesis of polyether antibiotics
2001.9-2002.3 Prof. Bao Xiaoming's labDepartment of MicrobiologyShandong UniversityYeast's Genetics
2000.3-2001.9 Prof. Wang Yuping's labDepartment of MicrobiologyShandong UniversityBiodegradation of cellulose and lignin

Research capabilities:

Molecular Cloning

Include:

DNA/RNA isolation and purification
PCR and Clone target fragment
RT-PCR
Construction of the genome library by cosmid
Southern hybridization by isotope
Site-directed mutagenesis by PCR
Practical Streptomyces Genetics
Practically in Streptomyces nanchangensis NS3226 and S. lividans 1326
Protein expression in E.coli
Protein purification by FPLC
HPLC (Waters 2960)
Preparative HPLC (SHIMADZU)
LC-ESI, Agilent 1100 to detect the Nanchangmycin, Meilingmycin and other small molecular

Publication:
T Liu, D You, C Valenzano, Y Sun, J Li, Q Yu, X Zhou, D. Cane, Z Deng (2006): Identification of NanE as the Thioesterase for Polyether Chain Release in Nanchangmycin Biosynthesis, Chemistry & Biology, 13(9):945-55. [Abstract]