AEM: HTS for antibiotic biosynthesis activators

Appl Environ Microbiol. 2012 Jun;78(12):4526-8. doi: 10.1128/AEM.00348-12.

High-throughput screening for Streptomyces antibiotic biosynthesis activators.

Chen L, Wang Y, Guo H, Xu M, Deng Z, Tao M.

 

 

 

 

Link to PubMed

 

A genomic cosmid library of Streptomyces clavuligerus was constructed and transferred efficiently by conjugation to Streptomyces lividans, and 12 distinct groups of overlapping cosmid clones that activated the silent actinorhodin biosynthesis gene cluster were identified. This generally applicable high-throughput screening procedure greatly facilitates the identification of antibiotic biosynthesis activators.

 

Copyright © 2016,The Laboratory of Molecular Microbiology at Shanghai Jiao Tong University. All rights reserved.

Add:Science Building, Xuhui Campus, Shanghai Jiaotong University, Shanghai 200030, China.   Tel:021-62932943