Shuangjun Lin

Mechanism of Natural Products Biosynthesis



Dr. Shuangjun Lin received his Ph.D degree in organic chemistry at the Institute of Chemistry, Chinese Academy of Sciences in 2002. He had postdoctoral research experience at University of Alberta in Canada and University of Wisconsin-Madison from 2002 to 2008, respectively. He was appointed a professor in Shanghai Jiao Tong University since the end of 2008. His current research is focused on the discovery of bioactive natural products from microbes and natural product biosynthesis.
Research Direction

(1)  Biosynthesis of natural products and synthetic biology.

Fig 1  Characterization of Streptonigrin Biosynthesis Reveals a Cryptic Carboxyl Methylation and an Unusual Oxidative Cleavage of a N-C Bond (Wu, et al. J Am Chem Soc, 2013)

(2)  Discovery and structural elucidation of biologically active natural products from special microorganisms.

 Fig 2  Organization of the pyridomycin biosynthetic gene cluster and model for the biosynthesis of pyridomycin. (Huang, et al. J Biol Chem. 2011)

(3)  Enzymatic mechanism and application of enzymes in organic synthesis.

Fig 3  A Trans-acting Ketoreductase in Biosynthesis of a Symmetric Polyketide Dimer SIA7248 (Zou, et al. Chembiochem. 2013)



2002.08    Ph.D. degree in organic chemistry. Institute of Chemistry, Chinese Academy of Sciences
1999.07    M.Sc. degree in organic chemistry, Shanghai Institute of Materia Medica, Chinese Academy of Sciences
1996.07    B. Sc. degree in chemistry, Ji Lin University


Research experiences

2008.11-present    Professor School of Life Sciences and Biotechnology, Shanghai Jiao University
2005.09-2008.09  Research Associate School of Pharmacy, University of Wisconsin-Madison, USA
2002.12-2005.08  Postdoctoral Fellow Department of Chemistry, University of Alberta, Canada
2002.09-2002.11  Assistant The Institute of Chemistry, CAS
Selected Publications
1. Kong D, Lee MJ, Lin S*, Kim ES*. Biosynthesis and Pathway Engineering of Antifungal Polyene Macrolides in Actinomycetes. J Ind Microbiol Biotechnol. 2013 Mar 21. [Epub ahead of print].
2. Lin S, Huang T, Shen B*. Tailoring enzymes acting on carrier protein-tethered substrates in natural product biosynthesis. Methods Enzymol. 2012, 516, 321-43.
3. Lin S, Huang T, Horsman GP, Huang SX, Guo X, Shen B*. Specificity of the ester bond forming condensation enzyme SgcC5 in C-1027 biosynthesis. Org Lett. 2012, 14, 2300-3.
4. Huang T, Wang Y, Yin J, Du Y, Tao M, Xu J, Chen W, Lin S*, Deng Z. Identification and characterization of the pyridomycin biosynthetic gene cluster of Streptomyces pyridomyceticus NRRL B-2517. J Biol Chem. 2011, 286, 20648-57.
5. Lin S, Horsman GP, Shen B*. Characterization of the epoxide hydrolase NcsF2 from the neocarzinostatin biosynthetic gene cluster. Org Lett. 2010, 12, 3816-9.
6. Lin S, Horsman GP, Chen Y, Li W, Shen B*. Characterization of the SgcF epoxide hydrolase supporting an (R)-vicinal diol intermediate for enediyne antitumor antibiotic C-1027 biosynthesis. J Am Chem Soc. 2009,131, 16410-7. 7.
7. Lin S, van Lanen SG, Shen B*. A free-standing condensation enzyme catalyzing ester bond Formation in C-1027 Biosynthesis. PNAS 2009, 106,4183-8.
8. Lin S, Van Lanen SG and Shen B*. “Regiospecific Chlorination of (S)-b-Tyrosyl-S -Carrier Protein Catalyzed by SgcC3 in the Biosynthesis of the Enediyne Antitumor Antibiotic C-1027”. J. Am. Chem. Soc.2007, 129, 12432-8.
9. Lin S,Van Lanen SG and Shen B*. “Characterization of the Two-Component, FAD- Dependent Monooxygenase SgcC that Requires Carrier Protein-Tethered Substrates for the Biosynthesis of the Enediyne Antitumor Antibiotic C-1027”. J. Am. Chem. Soc.2008, 130, 6616-6623.
10. Van Lanen SG, Lin S and Shen B*. “The Biosynthesis of the Enediyne Antitumor Antibiotic C-1027 Involves a New Branching Point in Chorismate Metabolism” PNAS. 2008, 105, 494-499.
11. Luo YG, Lin S, Zhang J. Cooke HA, Bruner SD, and Shen B. “Regiospecific O- methylation of naphthoic acids catalyzed by NcsB1, an O-methyltransferase involved in the biosynthesis of the enediyne antitumor antibiotic neocarzinostatin” J. Biol. Chem. 2008, 283, 14694-14702
12. Greco A, Ho JGS, Lin SJ, Palcic MM, Rupnik M, Ng KKS, “Carbohydrate recognition by Clostridium difficile toxin A” Nature Structural & Molecular Biology 2006, 13, 460-1.
13. Wang MX*, Lin SJ, Liu J, et al. “Efficient biocatalytic synthesis of highly enantiopure alpha-alkylated  arylglycines and amides” Advanced Synthesis & Catalysis. 2004,346, 439-45.
14. Wang MX*, Lin SJ, Liu CS, et al. “Nitrile biotransformations for highly efficient and enantioselective syntheses of electrophilic oxiranecarboxamides” J. Org. Chem. 2003, 68, 4570-3.
15. Wang MX*, Lin SJ. “Practical and convenient enzymatic synthesis of enantiopure alpha-amino acids and amides” J. Org. Chem. 2002, 67, 6542-5.
16. Lin SJ, Jiang SH, Zhu DY*, et al. “Two novel iridoids from Scrophularia buergeriana . Tetrahedron Lett. 2000, 41, 1069-71.

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